The protein associated with hairless gene is known as “hairless protein”, which is necessary for hair growth and when it stops functioning then complete hairlessness will occur. This gene is located on Chromosome 8 at position 22027873-22045326. Hairless gene comes under the super family of JmjC domain containing proteins and also functions in the mechanism of histone demethylation. The length of domain sequence is 212 amino acids which is present within the hairless protein of 1189 residues, from residue position 946 to 1157. In more than 100 eukaryotic and bacterial sequences, JmjC domains have been identified on the basis of significant sequence similarity, which include human hairless gene, mutated in individuals with alopecia universalis. We have attempted the bioinformatics approach to homology model the JmjC domain in the hairless protein. The tools and softwares used in this work are NCBI-BLASTP, EBIClustalW, SMART, 3D-PSSM, DeepView /Swiss–PDB Viewer, PyMOL and WhatCheck. The structure of JmjC domain is predicted by using the template crystal structure of probable antibiotic synthesis protein from Thermus thermophilus HB8. The minimized energy value of modeled domain structure was -3394.570 KJ/mol. WHAT IF-Proteins Model Check tool was used in the validation of modelled domain structure.